Onsite infectious agents and toxins monitoring in 12 May Sichuan earthquake affected areas
Identifieur interne : 002C25 ( Main/Exploration ); précédent : 002C24; suivant : 002C26Onsite infectious agents and toxins monitoring in 12 May Sichuan earthquake affected areas
Auteurs : Maosheng Yao [République populaire de Chine] ; Tong Zhu [République populaire de Chine] ; Kejun Li [République populaire de Chine] ; Shuofei Dong [République populaire de Chine] ; Yan Wu [République populaire de Chine] ; Xinghua Qiu [République populaire de Chine] ; Bo Jiang [République populaire de Chine] ; Liansheng Chen [République populaire de Chine] ; Shiqi Zhen [République populaire de Chine]Source :
- Journal of Environmental Monitoring [ 1464-0325 ] ; 2009.
English descriptors
- Teeft :
- Airborne, Airborne bacteria, Airborne concentration, Airborne virus, Allergen, Allergen concentrations, Aspergillus fumigatus, Assay, Avian virus, Bacillus anthracis, Bacterial concentrations, Bioaerosol, Bioaerosol sampling, Bioaerosol sampling tools, Biological agents, Bordetella pertussis, Button aerosol sampler, Concerted effort, Dead bodies, Detection limits, Devastating earthquake, Different sites, Disaster areas, Disaster sites, Disease outbreaks, Disease vectors, Diseases prevention, Dutch expert committee, Earthquake, Earthquake areas, Effective monitoring, Electrophoresis tests, Electrostatic collection, Elisa, Endotoxin, Endotoxin concentrations, Endotoxin levels, Enormous risk, Enteric viruses, Environ, Environmental endotoxin, Environmental toxins, Epidemic outbreak, Error bars, Evacuation measures, Exposure limit, Gelatin, Gelatin suspension, Gene copies, Glucan, Glucan concentrations, Glucan levels, Glucatell assay, Health council, Health effects, Health perspect, Health services, High concentration rate, High flow, High levels, High volume bioaerosol sampler, Higher levels, Hospital rooms, Human lives, Inadequate shelter, Infectious agents, Infectious disease, Infectious disease outbreak, Infectious disease outbreaks, Infectious diseases, Large scale, Lower sampling, Lowest standards, Lter, Lter samples, Mainelis, Major disaster, Major earthquake, Medical center, Medical centers, Meningococcal meningitis, Military tents, Mobile laboratory, Molecular devices, Monit, Monitoring work, Mycobacterium tuberculosis, National science foundation, Negative control, Negative controls, Neisseria meningitidis, Occupational standards, Onsite monitoring, Other hand, Other sites, Outbreak, Peking university, Polymerase chain reaction, Poor access, Positive control, Potential sources, Primer, Qpcr, Qpcr technique, Qpcr tests, Recent study, Refugee camps, Refugee tents, Relevant health authorities, Rescue worker camps, Rescue worker tents, Rescue workers, Respiratory disease outbreak, Richter scale, Royal society, Rubble, Sample processing, Sampler, Sampling scheme, Sichuan, Sichuan disaster areas, Sichuan earthquake, Sichuan earthquake areas, Sichuan province, Sichuan university, Sodium hypochlorite, Standard deviation, Standard series, Sybr qpcr tests, Times dilution, Troop camps, Viral indicators, Woods hole.
Abstract
At 14:28 on 12 May 2008, Sichuan Province of China suffered a devastating earthquake measuring 8.0 on the Richter scale with more than 80000 human lives lost and millions displaced. With inadequate shelter, poor access to health services, and disrupted ecology, the survivors were at enormous risk of infectious disease outbreaks. This work, believed to be unprecedented, was carried out to contain a possible outbreak through onsite monitoring of airborne biological agents in the high-risk areas. In such a mission, a mobile laboratory was developed using a customized vehicle along with state-of-art bioaerosol and molecular equipment and tools, and deployed to Sichuan 11 days after the earthquake. Using a high volume bioaerosol sampler (RCS High Flow) and Button Inhalable Aerosol Sampler equipped with gelatin filters, a total of 55 air samples, among which are 28 filter samples, were collected from rubble, medical centers, and camps of refugees, troops and rescue workers between 23 May and 9 June, 2008. After pre-treatment of the air samples, quantitative polymerase chain reaction (qPCR), gel electrophoresis, limulus amebocyte lysate (LAL) assay and enzyme-linked immunosorbent assay (ELISA) were applied to detect infectious agents and to quantify environmental toxins and allergens. The results revealed that, while high levels of endotoxin (180975 ng/m3) and (1,3)--d-glucans (11100 ng/m3) were observed, infectious agents such as Bacillus anthracis, Bordetella pertussis, Neisseria meningitidis, Mycobacterium tuberculosis, influenza A virus, bird flu virus (H5N1), enteric viruses, and Meningococcal meningitis were found below their detection limits. The total bacterial concentrations were found to range from 250 to 2.5 105 DNA copies/L. Aspergillus fumigatus (Asp f 1) and dust mite allergens (Der p 1 and Der f 1) were also found below their detection limits.
Url:
DOI: 10.1039/b910243d
Affiliations:
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sortKey="Chen, Liansheng" sort="Chen, Liansheng" uniqKey="Chen L" first="Liansheng" last="Chen">Liansheng Chen</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Centers for Diseases Prevention and Control, 210009, Jiangsu, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author wicri:is="90%"><name sortKey="Zhen, Shiqi" sort="Zhen, Shiqi" uniqKey="Zhen S" first="Shiqi" last="Zhen">Shiqi Zhen</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Centers for Diseases Prevention and Control, 210009, Jiangsu, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Environmental Monitoring</title><title level="j" type="abbrev">J. Environ. Monit.</title><idno type="ISSN">1464-0325</idno><idno type="eISSN">1464-0333</idno><imprint><publisher>The Royal Society of Chemistry.</publisher><date type="published" when="2009">2009</date><biblScope unit="volume">11</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1993">1993</biblScope><biblScope unit="page" to="2001">2001</biblScope></imprint><idno type="ISSN">1464-0325</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1464-0325</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Airborne</term><term>Airborne bacteria</term><term>Airborne concentration</term><term>Airborne virus</term><term>Allergen</term><term>Allergen concentrations</term><term>Aspergillus fumigatus</term><term>Assay</term><term>Avian virus</term><term>Bacillus anthracis</term><term>Bacterial concentrations</term><term>Bioaerosol</term><term>Bioaerosol sampling</term><term>Bioaerosol sampling tools</term><term>Biological agents</term><term>Bordetella pertussis</term><term>Button aerosol sampler</term><term>Concerted effort</term><term>Dead bodies</term><term>Detection limits</term><term>Devastating earthquake</term><term>Different sites</term><term>Disaster areas</term><term>Disaster sites</term><term>Disease outbreaks</term><term>Disease vectors</term><term>Diseases prevention</term><term>Dutch expert committee</term><term>Earthquake</term><term>Earthquake areas</term><term>Effective monitoring</term><term>Electrophoresis tests</term><term>Electrostatic collection</term><term>Elisa</term><term>Endotoxin</term><term>Endotoxin concentrations</term><term>Endotoxin levels</term><term>Enormous risk</term><term>Enteric viruses</term><term>Environ</term><term>Environmental endotoxin</term><term>Environmental toxins</term><term>Epidemic outbreak</term><term>Error bars</term><term>Evacuation measures</term><term>Exposure limit</term><term>Gelatin</term><term>Gelatin suspension</term><term>Gene copies</term><term>Glucan</term><term>Glucan concentrations</term><term>Glucan levels</term><term>Glucatell assay</term><term>Health council</term><term>Health effects</term><term>Health perspect</term><term>Health services</term><term>High concentration rate</term><term>High flow</term><term>High levels</term><term>High volume bioaerosol sampler</term><term>Higher levels</term><term>Hospital rooms</term><term>Human lives</term><term>Inadequate shelter</term><term>Infectious agents</term><term>Infectious disease</term><term>Infectious disease outbreak</term><term>Infectious disease outbreaks</term><term>Infectious diseases</term><term>Large scale</term><term>Lower sampling</term><term>Lowest standards</term><term>Lter</term><term>Lter samples</term><term>Mainelis</term><term>Major disaster</term><term>Major earthquake</term><term>Medical center</term><term>Medical centers</term><term>Meningococcal meningitis</term><term>Military tents</term><term>Mobile laboratory</term><term>Molecular devices</term><term>Monit</term><term>Monitoring work</term><term>Mycobacterium tuberculosis</term><term>National science foundation</term><term>Negative control</term><term>Negative controls</term><term>Neisseria meningitidis</term><term>Occupational standards</term><term>Onsite monitoring</term><term>Other hand</term><term>Other sites</term><term>Outbreak</term><term>Peking university</term><term>Polymerase chain reaction</term><term>Poor access</term><term>Positive control</term><term>Potential sources</term><term>Primer</term><term>Qpcr</term><term>Qpcr technique</term><term>Qpcr tests</term><term>Recent study</term><term>Refugee camps</term><term>Refugee tents</term><term>Relevant health authorities</term><term>Rescue worker camps</term><term>Rescue worker tents</term><term>Rescue workers</term><term>Respiratory disease outbreak</term><term>Richter scale</term><term>Royal society</term><term>Rubble</term><term>Sample processing</term><term>Sampler</term><term>Sampling scheme</term><term>Sichuan</term><term>Sichuan disaster areas</term><term>Sichuan earthquake</term><term>Sichuan earthquake areas</term><term>Sichuan province</term><term>Sichuan university</term><term>Sodium hypochlorite</term><term>Standard deviation</term><term>Standard series</term><term>Sybr qpcr tests</term><term>Times dilution</term><term>Troop camps</term><term>Viral indicators</term><term>Woods hole</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">At 14:28 on 12 May 2008, Sichuan Province of China suffered a devastating earthquake measuring 8.0 on the Richter scale with more than 80000 human lives lost and millions displaced. With inadequate shelter, poor access to health services, and disrupted ecology, the survivors were at enormous risk of infectious disease outbreaks. This work, believed to be unprecedented, was carried out to contain a possible outbreak through onsite monitoring of airborne biological agents in the high-risk areas. In such a mission, a mobile laboratory was developed using a customized vehicle along with state-of-art bioaerosol and molecular equipment and tools, and deployed to Sichuan 11 days after the earthquake. Using a high volume bioaerosol sampler (RCS High Flow) and Button Inhalable Aerosol Sampler equipped with gelatin filters, a total of 55 air samples, among which are 28 filter samples, were collected from rubble, medical centers, and camps of refugees, troops and rescue workers between 23 May and 9 June, 2008. After pre-treatment of the air samples, quantitative polymerase chain reaction (qPCR), gel electrophoresis, limulus amebocyte lysate (LAL) assay and enzyme-linked immunosorbent assay (ELISA) were applied to detect infectious agents and to quantify environmental toxins and allergens. The results revealed that, while high levels of endotoxin (180975 ng/m3) and (1,3)--d-glucans (11100 ng/m3) were observed, infectious agents such as Bacillus anthracis, Bordetella pertussis, Neisseria meningitidis, Mycobacterium tuberculosis, influenza A virus, bird flu virus (H5N1), enteric viruses, and Meningococcal meningitis were found below their detection limits. The total bacterial concentrations were found to range from 250 to 2.5 105 DNA copies/L. Aspergillus fumigatus (Asp f 1) and dust mite allergens (Der p 1 and Der f 1) were also found below their detection limits.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Yao, Maosheng" sort="Yao, Maosheng" uniqKey="Yao M" first="Maosheng" last="Yao">Maosheng Yao</name></noRegion><name sortKey="Chen, Liansheng" sort="Chen, Liansheng" uniqKey="Chen L" first="Liansheng" last="Chen">Liansheng Chen</name><name sortKey="Dong, Shuofei" sort="Dong, Shuofei" uniqKey="Dong S" first="Shuofei" last="Dong">Shuofei Dong</name><name sortKey="Dong, Shuofei" sort="Dong, Shuofei" uniqKey="Dong S" first="Shuofei" last="Dong">Shuofei Dong</name><name sortKey="Jiang, Bo" sort="Jiang, Bo" uniqKey="Jiang B" first="Bo" last="Jiang">Bo Jiang</name><name sortKey="Li, Kejun" sort="Li, Kejun" uniqKey="Li K" first="Kejun" last="Li">Kejun Li</name><name sortKey="Li, Kejun" sort="Li, Kejun" uniqKey="Li K" first="Kejun" last="Li">Kejun Li</name><name sortKey="Qiu, Xinghua" sort="Qiu, Xinghua" uniqKey="Qiu X" first="Xinghua" last="Qiu">Xinghua Qiu</name><name sortKey="Qiu, Xinghua" sort="Qiu, Xinghua" uniqKey="Qiu X" first="Xinghua" last="Qiu">Xinghua Qiu</name><name sortKey="Wu, Yan" sort="Wu, Yan" uniqKey="Wu Y" first="Yan" last="Wu">Yan Wu</name><name sortKey="Wu, Yan" sort="Wu, Yan" uniqKey="Wu Y" first="Yan" last="Wu">Yan Wu</name><name sortKey="Yao, Maosheng" sort="Yao, Maosheng" uniqKey="Yao M" first="Maosheng" last="Yao">Maosheng Yao</name><name sortKey="Zhen, Shiqi" sort="Zhen, Shiqi" uniqKey="Zhen S" first="Shiqi" last="Zhen">Shiqi Zhen</name><name sortKey="Zhu, Tong" sort="Zhu, Tong" uniqKey="Zhu T" first="Tong" last="Zhu">Tong Zhu</name><name sortKey="Zhu, Tong" sort="Zhu, Tong" uniqKey="Zhu T" first="Tong" last="Zhu">Tong Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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